ADIPOR1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA048306

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA048306 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

67 

Antigen sequence

HKGSVVAQGNGAPASNREADTVELAELGPLLEEKGKRVIANPPKAEEEQT
CPVPQEEEEEVRVLTLP
 

Matching transcripts

ADIPOR1-001 - ENSP00000341785 [100%]
ADIPOR1-002 - ENSP00000402178 [100%]
ADIPOR1-003 - ENSP00000392946 [100%]
ADIPOR1-201 - ENSP00000356223 [100%]
ADIPOR1-202 - ENSP00000395469 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli & cytoplasm.
More information
 

Antibody dilution

1:115 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

42.6, 30.6, 23.6, 19.3 

Antibody dilution

1:570 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:4600 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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ADIPOR1-001
 
ADIPOR1-002
 
ADIPOR1-003
 
ADIPOR1-201
 
ADIPOR1-202