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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Cytoplasm, Mitochondria

Additional location(s)

 

Staining summary

Localized to the cytoplasm, nucleoli and mitochondria.

Reliability (APE)

Very low

Antibodies in assay

HPA010641, HPA014147
 

Antibody HPA010641

 

Antibody HPA014147

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm Mitochondria 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

Cluster  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Mitochondria 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in mitochondria.
 

Summary

Staining of cytoplasm and nucleoli in all three cell lines.   Staining of mitochondria in U-251 MG and A-431. Staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleoli