Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Plasma membrane

Additional location(s)

Cytoplasm, Cytoskeleton (Actin filaments) 

Staining summary

Staining of nucleus, plasma membrane, cytoplasm and cytoskeleton (actin filaments) in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA038267
 

Antibody HPA038267

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

 

Staining characteristics

 

Staining intensity

Negative 

Validation

No staining.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of nucleus, plasma membrane, cytoplasm and cytoskeleton (actin filaments) in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Actin filaments
Nucleus but not nucleoli
Plasma membrane