The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cytoplasmic and nuclear expression in several tissue types. Most abundant in CNS, breast, salivary glands, melaocytes, germinal center cells of tonsil and lymph node.
Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human hippocampus shows nuclear and cytoplasmic positivity in subsets of glial cells.
Summary
Strong nuclear and cytoplasmic positivity was observed subset of cells in epidermis and tonsil. Several glandular epithelia were moderately stained, while squamous epithelia generally were negative.
Normal tissues generally displayed cytoplasmic staining of moderate to strong intensity, often in combination with positivity of a few nuclei. Strongest immunoreactivity was observed in heart muscle, astrocytes and cells of thyroid gland, breast and salivary gland. Chondrocytes stained distinctly.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
