Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the nucleoli.

Reliability (APE)

Medium

Antibodies in assay

HPA001844, CAB005374, CAB016305
 

Antibody HPA001844

 

Antibody CAB005374

 

Antibody CAB016305

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm   Cytoplasm 

Staining characteristics

Spotty   Granular 

Staining intensity

Moderate   Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
   
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm Cytoplasm  

Staining characteristics

   

Staining intensity

Moderate Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Cytoplasm  

Staining characteristics

Spotty   

Staining intensity

Moderate Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm and spots in nucleoli in all three cell lines.   Staining of cytoplasm in U-251 MG and U-2 OS.   Granular staining of the cytoplasm in a subset of A-431 cells.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli