PTH1R

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA007978

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA007978 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

111 

Antigen sequence

RWTLALDFKRKARSGSSSYSYGPMVSHTSVTNVGPRVGLGLPLSPRLLPT
ATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNGSCSGLDEEA
SGPERPPALLQ
 

Matching transcripts

PTH1R-001 - ENSP00000321999 [100%]
PTH1R-002 - ENSP00000402723 [100%]
PTH1R-003 - ENSP00000411424 [100%]
PTH1R-008 - ENSP00000396176 [100%]
PTH1R-201 - ENSP00000413774 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows moderate positivity in a subset of tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:100 

Literature conformity

Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not consistent with RNA expression data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

66.4, 20.0 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PTH1R-001
 
PTH1R-002
 
PTH1R-003
 
PTH1R-008
 
PTH1R-201