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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Mainly localized to the nucleus and the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

CAB005196, HPA027273, HPA027369, HPA028251
 

Antibody CAB005196

 

Antibody HPA027273

 

Antibody HPA027369

 

Antibody HPA028251

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli Cytoplasm 

Staining characteristics

  Speckle   

Staining intensity

  Moderate Moderate Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

     

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

 Speckle  Granular 

Staining intensity

Negative Strong Strong Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli Nucleus but not nucleoli Cytoplasm 

Staining characteristics

 Speckle   

Staining intensity

Negative Strong Moderate Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Speckled staining of the nucleus in all three cell lines with additional staining of cytoplasm in A-431.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-2 OS.   Staining of the cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli