Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus. In addition localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB008649, HPA023225
 

Antibody CAB008649

 

Antibody HPA023225

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus