Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of cytoplasm in all three cell lines. Additional staining of nucleus in A-431 and of cytoskeleton (intermediate filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA027002
 

Antibody HPA027002

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of nucleus in A-431 and of cytoskeleton (intermediate filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Nucleus but not nucleoli