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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Nuclear membrane

Additional location(s)

Plasma membrane, Vesicles, Cytoskeleton (Intermediate filaments) 

Staining summary

Mainly localized to the nucleus and nuclear membrane. Additionally localized to vesicles and intermediate filaments.

Reliability (APE)

Medium

Antibodies in assay

HPA018410, HPA018441
 

Antibody HPA018410

 

Antibody HPA018441

 

         Cell line A-431

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Location(s)

Nuclear membrane, Vesicles Nucleus but not nucleoli, Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nuclear membrane, Vesicles, Cytoskeleton (Intermediate filaments) Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nuclear membrane, Cytoskeleton (Intermediate filaments) Nucleus but not nucleoli, Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane, vesicles & cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & nuclear membrane.
 

Summary

Staining of nuclear membrane in all three cell lines. Additional staining of cytoskeleton (intermediate filaments) in U-251 MG, vesicles in A-431 and both in U-2 OS.   Staining of nucleus in all three cell lines with additional staining of nuclear membrane in U-251 MG and A-431 and plasma membrane in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Intermediate filaments
Nuclear membrane
Nucleus but not nucleoli
Plasma membrane
Endosome