Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Actin filaments)

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Staining of cytoskeleton (actin filaments) in all three cell lines, with additional staining of cytoplasm in MCF-7 and cytoplasm and nucleus in A-431.

Reliability (Single)

Antibodies in assay

HPA045778
 

Antibody HPA045778

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line MCF-7

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Location(s)

Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (actin filaments).
 

Summary

Staining of cytoskeleton (actin filaments) in all three cell lines, with additional staining of cytoplasm in MCF-7 and cytoplasm and nucleus in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Nucleus but not nucleoli