Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Mainly localized to the nucleus but also to the plasma membrane and cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA006735, HPA017142
 

Antibody HPA006735

 

Antibody HPA017142

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of nucleus in U-251MG and U-2 OS.   Staining of nuclei but not nucleoli in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane