The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Summary
Moderate staining was observed in most glandular cells. Remaining normal cells were mainly negative or weakly stained.
Moderate cytoplasmic and occasional membranous positivity was observed in parts of the gastrointestinal tract, thyroid, adrenal cortex, renal tubules and endometrial glands. Remaining normal tissues were weakly stained or negative.
Most normal tissues showed moderate cytoplasmic immunoreactivity. Additional membranous staining was observed in the prostate, parathyroid and uterine glands. Renal tubules showed strong positivity. Lung, renal glomeruli, myocytes and cells in CNS were weakly stained or negative.
Most normal tissues showed moderate to strong cytoplasmic positivity. Neuronal cells in CNS showed additional nuclear staining. Glial cells in CNS, lymphoid cells, smooth muscle, bile ducts, trophoblastic cells were generally negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
