Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nuclear membrane 

Staining summary

Mainly localized to the cytoplasm but also to the nuclear membrane.

Reliability (APE)

High

Antibodies in assay

HPA029700, HPA029702, HPA029703
 

Antibody HPA029700

 

Antibody HPA029702

 

Antibody HPA029703

 

         Cell line A-431

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Location(s)

 Nuclear membrane, Cytoplasm Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Moderate Weak 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm Cytoplasm 

Staining characteristics

   

Staining intensity

Weak Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nuclear membrane, Cytoplasm Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in U-2 OS.   Staining of cytoplasm in all three cell lines. Additional staining of the nuclear membrane in U-251 MG and A-431.   Staining of cytoplasm in all thre cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nuclear membrane