The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human lymph node shows strong positivity in subsets of cells outside the reaction center.
Summary
Strong staining was observed in apoptotic bodies in lymphoid tissue. Cytoplasmic staining, weak to moderate, was found in glandular cells in intestine, male genitalia, liver and kidney. Remaining cells were weakly stained or negative.
Distinct cytoplasmic positivity was observed in reaction centra of lymphoid tissues, in bone marrow, in plasma cells and in glandular epithelia of the gastrointestinal tract. Remaining normal tissues were negative.
Weak to moderate cytoplasmic staining in most normal tissues. Respiratory epithelia and lymphoid tissues showed moderate to strong cytoplasmic or nuclear staining. Cytotrophoblasts in the placenta showed stronger nuclear and cytoplasmic staining than syncytiotrophoblasts. Pyramidal neurons of the hippocampus showed distinct cytoplasmic staining.
Validation
One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
