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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria

Additional location(s)

 

Staining summary

Staining of cytoplasm, mitochondria and nucleus in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA002643
 

Antibody HPA002643

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & mitochondria.
 

Summary

Staining of cytoplasm, mitochondria and nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli