The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in subsets of cells in the red pulp.
Summary
Most of the normal tissues displayed moderate cytoplasmic staining with a granular pattern, in several cases combined with membranous positivity. Airway epithelia, breast, basal cells in prostate and seminiferous ducts as well as subsets of lymphoid cells were strongly stained. Bile duct cells, cardiac and smooth muscle cells were weakly stained or negative.
Subsets of cells in red pulp of spleen and bone marrow poietic cells showed moderate cytoplasmic staining. Heart muscle displayed staining in intercalated discs while the adrenal gland showed moderate cytoplasmic and nuclear positivity. Remaining normal tissues were weakly stained or negative.
Validation
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
