The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Selective cytoplasmic and nuclear expression in squamous epithelia, urothelia, stomach, large intestine, adipocytes, endothelial cells and subsets of lymphoid cells.
Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in epidermal cells.
Summary
Squamous epithelia and urothelium displayed strong cytoplasmic and nuclear positivity. Weak to moderate cytoplasmic staining was observed in the gastrointestinal tract and lymphoid tissues. Remaining normal tissues were generally negative.
Squamous epithelia, the gastrointestinal tract, urothelium and endothelial cells displayed strong cytoplasmic and nuclear immunoreactivity. Lymphoid reaction center cells, fractions of cells in CNS and seminiferus ducts displayed moderate cytoplasmic and nuclear staining. Remaining normal cells were generally negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
