COX6C

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA014295

 

Antibody CAB016244

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA014295 sc-65240 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

12 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

28  

Antigen sequence

EPRKKAYADFYRNYDVMKDFEDMRKTGT
 
 

Matching transcripts

COX6C-001 - ENSP00000428895 [82%]
COX6C-002 - ENSP00000429755 [82%]
COX6C-003 - ENSP00000297564 [82%]
COX6C-004 - ENSP00000429707 [82%]
COX6C-005 - ENSP00000429714 [82%]
COX6C-006 - ENSP00000428965 [82%]
COX6C-007 - ENSP00000428150 [82%]
COX6C-008 - ENSP00000429410 [82%]
COX6C-009 - ENSP00000429991 [82%]
COX6C-010 - ENSP00000428702 [82%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
More information
 

Antibody dilution

1:100 1:75 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

8.8 8.8 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human corpus, uterine shows granular cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:300 

Validation IH

 Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 
 

ANTIGEN VIEW

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COX6C-001
 
COX6C-002
 
COX6C-003
 
COX6C-004
 
COX6C-005
 
COX6C-006
 
COX6C-007
 
COX6C-008
 
COX6C-009
 
COX6C-010