ASB11

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA000238

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA000238 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

106 

Antigen sequence

YQRVDCVKKLLELGASVDHGQWLDTPLHAAARQSNVEVIHLLTDYGANLK
RRNAQGKSALDLAAPKSSVEQALLLREGPPALSQLCRLCVRKCLGRACHQ
AIHKLH
 

Matching transcripts

ASB11-001 - ENSP00000417914 [100%]
ASB11-003 - ENSP00000343408 [100%]
ASB11-004 - ENSP00000369837 [100%]
ASB11-201 - ENSP00000445465 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human liver shows nuclear positivity in hepatocytes.
More information
 

Retrieval method

HIER pH9 

Antibody dilution

1:150 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

35.4, 33.5, 33.0 

Antibody dilution

1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ASB11-001
 
ASB11-003
 
ASB11-004
 
ASB11-201