CLDN3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA007322

 

Antibody HPA014361

 

Antibody CAB002702

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Lab Vision/NeoMarkers
 

Product name

HPA007322 HPA014361 RB-9251 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

5 11 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Not known 

Length (aa)

29 35  

Antigen sequence

GLWMNCVVQSTGQMQCKVYDSLLALPQDL
 
PREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV
 
 

Matching transcripts

CLDN3-001 - ENSP00000378577 [100%]
 CLDN3-001 - ENSP00000378577 [100%]
  

Other gene match

CLDN6 - ENSG00000184697 [97%]
CLDN4 - ENSG00000189143 [100%]
CLDN9 - ENSG00000213937 [97%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

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Description

Immunohistochemical staining of human colon shows strong membranous and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human skin shows strong membranous positivity in epidermal cells.
More information
 Immunohistochemical staining of human small intestine shows strong positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:15 1:10 1:75 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

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Description

  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

23.3, 22.8, 22.1 23.3 23.3 

Antibody dilution

1:250 1:250 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

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Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:500 1:500  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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CLDN3-001