ATP7A

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA012887

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA012887 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

122 

Antigen sequence

TETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQID
ASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVND
FMTEHERKGRTAVLVAVDDELC
 

Matching transcripts

ATP7A-001 - ENSP00000345728 [100%]
ATP7A-201 - ENSP00000343026 [100%]
ATP7A-202 - ENSP00000343678 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and glial cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:10 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Western Blot

Image

 

Description

 

Target mass (kDa)

163.4, 154.4, 54.3 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ATP7A-001
 
ATP7A-201
 
ATP7A-202