The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Summary
A majority of normal cells displayed moderate to strong cytoplasmic and membranous positivity. Cells in CNS and lymphoid tissues were generally negative.
Ciliated cells in airway epithelia, epididymis and fallopian tube displayed strong membranous positivity.
Remaining normal cells showed weak to moderate staining. Squamous epithelia cells and most lymphoid tissues were negative.
Most normal cells showed moderate to strong cytoplasmic positivity with additional nuclear staining in a few cases. Subsets of lymphoid cells were weakly stained or negative.
Validation
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data
