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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli, Mitochondria

Additional location(s)

 

Staining summary

Localized to the nucleus or mitochondria and nucleoli.

Reliability (APE)

Very low

Antibodies in assay

HPA021443, HPA023894
 

Antibody HPA021443

 

Antibody HPA023894

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli & mitochondria.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG.   Staining of mitochondria and nucleoli in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Mitochondria
Nucleoli
Nucleus but not nucleoli