CACNB2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035326

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035326 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

80 

Antigen sequence

RDSAYVEPKEDYSHDHVDHYASHRDHNHRDETHGSSDHRHRESRHRSRDV
DREQDHNECNKQRSRHKSKDRYCEKDGEVI
 

Matching transcripts

CACNB2-001 - ENSP00000320025 [100%]
CACNB2-002 - ENSP00000282343 [100%]
CACNB2-003 - ENSP00000379821 [100%]
CACNB2-004 - ENSP00000366532 [100%]
CACNB2-005 - ENSP00000366536 [100%]
CACNB2-007 - ENSP00000366546 [100%]
CACNB2-008 - ENSP00000344474 [100%]
CACNB2-009 - ENSP00000366545 [100%]
CACNB2-010 - ENSP00000366548 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows moderate membranous positivity in islet cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:200 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

73.6, 71.1, 70.8, 68.8, 68.3, 68.2, 68.1, 64.0, 45.8 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CACNB2-001
 
CACNB2-002
 
CACNB2-003
 
CACNB2-004
 
CACNB2-005
 
CACNB2-007
 
CACNB2-008
 
CACNB2-009
 
CACNB2-010