ABTB2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA020065

 

Antibody HPA030699

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA020065 HPA030699 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

73 73 

Antigen sequence

IPTTDILELLSAASLFQLDALQRHCEILCSQTLSMESAVNTYKYAKIHNA
PELALFCEGFFLKHMKALLEQDA
 
MVDTRISVRIHEYAAISLTACMENLVEEIRARVMASHSPDGGGAGGGEVS
AEALEMVINNDAELWGVLQPYEH
 

Matching transcripts

ABTB2-001 - ENSP00000410157 [100%]
ABTB2-201 - ENSP00000298992 [100%]
 ABTB2-001 - ENSP00000410157 [100%]
ABTB2-201 - ENSP00000298992 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
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Retrieval method

HIER pH6  

Antibody dilution

1:60  

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus.
More information
 Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:20 1:59 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

113.7, 93.3 113.7, 93.3 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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ABTB2-001
 
ABTB2-201