Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA042529, HPA042616
 

Antibody HPA042529

 

Antibody HPA042616

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Strong 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of the nucleus in all three cell lines with additional staining of cytoplasm in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli