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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to microtubules or nucleus.

Reliability (APE)

Very low

Antibodies in assay

HPA020332, HPA021600
 

Antibody HPA020332

 

Antibody HPA021600

 

         Cell line A-431

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Location(s)

Cytoskeleton (Microtubules) Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Microtubules) Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoskeleton (Microtubules) Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
 

Summary

Staining of cytoskeleton (microtubules) in all three cell lines.   Staining of nuclei but not nucleoli in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Microtubules
Nucleus but not nucleoli