CATSPER2

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA042709

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042709 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

68 

Antigen sequence

SESIGRLDWETLVHENLPGLMEMDQDDRVWPRDSLFRYFELLEKLQYNLE
ERKKLQEFAVQALMNLED
 

Matching transcripts

CATSPER2-001 - ENSP00000380088 [100%]
CATSPER2-002 - ENSP00000321463 [100%]
CATSPER2-004 - ENSP00000371180 [100%]
CATSPER2-201 - ENSP00000339137 [100%]
CATSPER2-013 - ENSP00000388242 [87%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human soft tissue shows strong positivity in adipocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:160 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & mitochondria.
More information
 

Antibody dilution

1:200 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

62.5, 62.0, 61.9, 8.7 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
CATSPER2-001
 
CATSPER2-002
 
CATSPER2-004
 
CATSPER2-013
 
CATSPER2-201