Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA011755, HPA011891
 

Antibody HPA011755

 

Antibody HPA011891

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

   

Staining intensity

Moderate   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

Granular   

Staining intensity

Moderate   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Nucleus but not nucleoli