Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Centrosome 

Staining summary

Mainly localized to the cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

CAB003759, HPA007925, HPA011212
 

Antibody CAB003759

 

Antibody HPA007925

 

Antibody HPA011212

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Cytoplasm, Centrosome Cytoplasm 

Staining characteristics

 Granular Granular 

Staining intensity

Negative Weak Moderate 

Validation

No staining.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

  Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Negative Negative Weak 

Validation

No staining.
 
The multi-targeting antibody not yielding a staining pattern.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

  Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Negative Negative Weak 

Validation

No staining.
 
The multi-targeting antibody not yielding a staining pattern.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm & centrosome.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

No staining was observed in all three cell lines.   Staining of centrosome, cytoplasm and nuclei in A-431.   Staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli