Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Vesicles

Additional location(s)

Cytoplasm, Cytoskeleton (Microtubules) 

Staining summary

Localized to the nucleus or to vesicles and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA007993, CAB024999
 

Antibody HPA007993

 

Antibody CAB024999

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Vesicles 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm, Vesicles, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Cytoplasm, Vesicles 

Staining characteristics

 Fibrous 

Staining intensity

Weak Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & vesicles.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-2 OS.   Staining of vesicles in all three cell lines with additional staining of cytoplasm in U-251 MG and cytoplasm as well as cytoskeleton (microtubules) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Microtubules
Nucleus but not nucleoli
Endosome