Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Nucleoli 

Staining summary

Mainly localized to the cytoplasm but also to nucleus and nucleoli.

Reliability (APE)

Low

Antibodies in assay

HPA021623, HPA023246, HPA023248
 

Antibody HPA021623

 

Antibody HPA023246

 

Antibody HPA023248

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Weak Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm Cytoplasm   

Staining characteristics

    

Staining intensity

Weak Moderate   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

    

         Cell line U-251 MG

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Location(s)

 Cytoplasm Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Moderate Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus, nucleoli & cytoplasm.
 

Summary

Staining of the nucleus and cytoplasm in A-431 and U-2 OS.   Staining of cytoplasm in all three cell lines.   Staining of nucleus, nucleoli and cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus