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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to cytoplasm or intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

HPA001885, CAB024863
 

Antibody HPA001885

 

Antibody CAB024863

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoskeleton (Intermediate filaments) 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Moderate   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Cell line U-251 MG

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Location(s)

   

Staining characteristics

   

Staining intensity

  Negative 

Validation

  
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoskeleton (intermediate filaments).
 

Summary

Staining of cytoplasm in U-2 OS and a fraction of the cells in A-431.   Staining of cytoskeleton (intermediate filaments) in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments