FTH1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA043650

 

Antibody CAB008623

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 AbFrontier
 

Product name

HPA043650 LF-PA0063 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

10 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

26  

Antigen sequence

ASTSQVRQNYHQDSEAAINRQINLEL
 
 

Matching transcripts

FTH1-001 - ENSP00000273550 [100%]
FTH1-002 - ENSP00000434403 [100%]
FTH1-004 - ENSP00000432223 [100%]
FTH1-005 - ENSP00000431659 [100%]
FTH1-006 - ENSP00000431575 [100%]
FTH1-007 - ENSP00000433470 [100%]
  

Other gene match

FTMT - ENSG00000181867 [81%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:200 1:6000 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

 Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoskeleton (microtubules).
More information
 

Antibody dilution

 1:2000 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

27.5, 21.2, 13.3, 11.6, 11.2, 9.1, 5.6 21.2, 17.7, 13.3, 12.9, 11.6, 11.2, 9.1, 6.7, 5.6 

Antibody dilution

1:250 1:500 

Validation WB

Uncertain: No bands detected Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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FTH1-001
 
FTH1-002
 
FTH1-004
 
FTH1-005
 
FTH1-006
 
FTH1-007