Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Nucleoli, Plasma membrane, Vesicles 

Staining summary

Staining of nucleus in all three cell lines. Additional staining of vesicles in A-431 and vesicles and plasma membrane in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA031131
 

Antibody HPA031131

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleoli, Nucleus but not nucleoli 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane & vesicles.
 

Summary

Staining of nucleus in all three cell lines. Additional staining of vesicles in A-431 and vesicles and plasma membrane in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleoli
Nucleus but not nucleoli
Plasma membrane
Endosome