ATG4B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB037195

 

ANTIBODY INFORMATION

Provider

SIGMA
 

Product name

A2981 

Host species

Rabbit 

Clonality

pAb 

Purity

Protein A/G 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Synthetic peptide 

Length (aa)

 

Antigen sequence

 

Matching transcripts

 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:250 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

44.3, 42.6, 42.4, 37.0, 35.9, 30.2, 24.8, 21.2, 16.6, 12.1, 10.2, 8.8, 7.7 

Antibody dilution

1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

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