The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human cerebral cortex shows nuclear and cytoplasmic positivity in neuronal cells and glial cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic and nuclear positivity.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Summary
Most normal tissues showed moderate to strong cytoplasmic and nuclear positivity.
Most normal tissues displayed moderate to strong cytoplasmic and nuclear positivity.
Ciliated epithelia of respiratory tract and fallopian tube were distinctly stained. Fractions of exocrine glandular cells of pancreas, cells in red pulp of spleen and bone marrow displayed strong cytoplasmic staining. Moderate cytoplasmic and nuclear staining was observed in thyroid and adrenal gland. Neuronal cells and soft tissues were weakly stained. Remaining normal cells were negative.
Validation
Two (or more) antibodies yielding dissimilar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
