ASPSCR1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026749

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026749 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

81 

Antigen sequence

GSSASAGQAAASAPLPLESGELSRGDLSRPEDADTSGPCCEHTQEKQSTR
APAAAPFVPFSGGGQRLGGPPGPTRPLTSSS
 

Matching transcripts

ASPSCR1-001 - ENSP00000302176 [100%]
ASPSCR1-002 - ENSP00000306625 [100%]
ASPSCR1-003 - ENSP00000462329 [100%]
ASPSCR1-004 - ENSP00000463992 [100%]
ASPSCR1-009 - ENSP00000463316 [100%]
ASPSCR1-014 - ENSP00000462218 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:500 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

70.0, 60.2, 54.5, 49.8, 38.4, 32.8 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ASPSCR1-001
 
ASPSCR1-002
 
ASPSCR1-003
 
ASPSCR1-004
 
ASPSCR1-009
 
ASPSCR1-014