The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in cytotrophoblasts.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Summary
Most normal tissues exhibited weak to moderate cytoplasmic and occasional nuclear staining. The lung displayed strong staining in alveolar cells type 2 while type 1 cells were negative. A fraction of neuronal cells and cells in cerebellum were strongly stained. Adipocytes were distinctly stained.
Cytoplasmic staining in most normal tissues of variable intensity. Strong staining in type II alveolar cells, neuronal cells and several glandular epithelia. In stomach, strongest staining in chief cells. Cytotrophoblasts of placenta were strongly positive in cytoplasm, while syncytiotrophoblasts displayed faint cytoplasmic immunoreactivity, accompanied with distinct microvillous staining. Lymphoid tissues showed distinct staining in germinal centers whilst non germinal center cells were mainly negative.
Most normal tissues showed moderate to strong cytoplasmic positivity. Glial cells, spleen and myocytes were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
