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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to cytoplasm and plasma membrane.

Reliability (APE)

High

Antibodies in assay

CAB005192, HPA006461
 

Antibody CAB005192

 

Antibody HPA006461

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

 Smooth 

Staining intensity

Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

 Smooth 

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm, Golgi apparatus 

Staining characteristics

 Smooth 

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of plasma membrane in A-431.   Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of the Golgi apparatus in U-251MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane