Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane

Additional location(s)

Cell Junctions 

Staining summary

Staining of the nucleus and plasma membrane in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA039711
 

Antibody HPA039711

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cell Junctions 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & plasma membrane.
 

Summary

Staining of the nucleus and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cell junctions
Nucleus but not nucleoli
Plasma membrane