CDH2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB000141

 

Antibody CAB018580

 

ANTIBODY INFORMATION

Provider

Zymed
 Santa Cruz Biotechnology
 

Product name

18-0224 sc-8424 

Host species

Mouse Mouse 

Clonality

mAb mAb 

Purity

Not known Protein A/G 

Released in version

1 5 

ANTIGEN INFORMATION

Antigen

Not known Not known 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.
More information
 Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:40 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cell junctions.
More information
  

Antibody dilution

1:154  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

99.8, 97.0, 21.9, 13.8, 8.5 99.8, 97.0, 21.9, 13.8, 8.5 

Antibody dilution

1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 
 

ANTIGEN VIEW

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 Not available