Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm but also present in the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA021176, HPA021177, HPA027736
 

Antibody HPA021176

 

Antibody HPA021177

 

Antibody HPA027736

 

         Cell line A-431

Toggle channels

 
 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

Toggle channels

 
 
 

Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

Toggle channels

 
 
 

Location(s)

Cytoplasm Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Granular  

Staining intensity

Moderate Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in A-431.   Staining of cytoplasm and nucleus but not nucleoli in all three cell lines.   Staining of cytoplasm and nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli