The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells (goblet cells).
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Summary
Strong cytoplasmic immunoreactivity was observed in parietal cells of stomach and in goblet cells of the gastrointestinal tract. Distinct positivity was observed in luminal membranes, cilia and microvilli of several tissues, for exampled placenta and epididymis. Cells of the CNS, squamous epithelia and muscle cells were in general negative. Remaining normal tissues showed weak to moderate cytoplasmic positivity.
Moderate cytoplasmic immunoreactivity in most normal tissues with additional membranous positivity in a few cases. Dot-like positivity was observed in lymphocytes and smooth muscle. Astrocytes and axonal projections displayed distinct positivity.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
