Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the plasma membrane but also to the cytoplasm in two of the cell lines.

Reliability (APE)

Low

Antibodies in assay

HPA010008, HPA010665
 

Antibody HPA010008

 

Antibody HPA010665

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm  

Staining characteristics

  

Staining intensity

Strong Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Staining of plasma membrane in all three cell lines with additional staining of cytoplasm in U-2 OS and A-431.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane