The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells and cells in molecular layer.
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.
Summary
Selective cytoplasmic staining in CNS and glomeruli. Most other tissues and cells were negative.
Strong cytoplasmic staining was observed in neuronal cells, glomeruli and peripheral nerves. Strong membranous positivity was observed in most intestinal glands. Most remaining normal tissues were weakly stained or negative.
The central nervous system exhibited strong nuclear and cytoplasmic staining. Glomerular cells displayed strong cytoplasmic and membranous staining. Peripheral nerves and neuropil were distinctly stained. A subset of respiratory epithelia was moderately stained. Remaining normal tissues were negative.
Moderate cytoplasmic positivity was displayed in peripheral nerve. Neurons and cells in molecular layer of cerebellum showed moderate to strong cytoplasmic and nuclear staining. Renal glomeruli showed moderate cytoplasmic and membranous staining. Remaining normal tissues were mainly negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
