Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm, Mitochondria, Cell Junctions 

Staining summary

Mainly localized to nucleus. In addition localized to cytoplasm, cell junctions and mitochondria.

Reliability (APE)

Low

Antibodies in assay

HPA001056, CAB005081
 

Antibody HPA001056

 

Antibody CAB005081

 

         Cell line A-431

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Location(s)

 Nucleus, Cytoplasm, Cell Junctions 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus, Mitochondria 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & mitochondria.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines. Additional staining of mitochondria in U-251 MG and U-2 OS, and cytoplasm and cell junctions in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cell junctions
Cytoplasm
Mitochondria
Nucleus