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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Mitochondria

Additional location(s)

Plasma membrane 

Staining summary

Localized to cytoplasm, mitochondria and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA008237, CAB017181
 

Antibody HPA008237

 

Antibody CAB017181

 

         Cell line A-431

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm, Mitochondria 

Staining characteristics

Granular  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of mitochondria in U-251 MG and U-2 OS. Additional staining of plasma membrane in A-431 and U-251 MG and of cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Plasma membrane