Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus, Cytoplasm

Additional location(s)

Plasma membrane 

Staining summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in A-431.

Reliability (Single)

Antibodies in assay

HPA018525
 

Antibody HPA018525

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Nucleus, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane